genetic diversity of different populations of microcerotermes diversus (isoptera:termitidae) using coii gene

نویسندگان

الهام اکبریان فیروزآبادی

دانشجوی سابق کارشناسی ارشد، دانشکده کشاورزی، دانشگاه شهید چمران اهواز بهزاد حبیب پور

استادیار، دانشکده کشاورزی، دانشگاه شهید چمران اهواز حمید گله داری

دانشیار، دانشکده کشاورزی، دانشگاه شهید چمران اهواز پرویز شیشه بر

استاد، دانشکده کشاورزی، دانشگاه شهید چمران اهواز

چکیده

microcerotermes diversus (silvestri) (isoptera: termitidae), an important wood pest in ahwaz (khuzestan, iran), causes serious economic damage to cellulosic products in buildings. to investigate the genetic diversity of this pest, 13 populations from different areas of khuzestan province as well as two samples from khark island and bandar abbas were collected. following dna extraction a fragment of 486 bp length from the mithochondrial cytochrome oxidase gene subunit ii (coii) was amplified and subsequently sequenced. the maximum parsimony analyses were done through mega4. the characteristics of the most parsimonious tree were recorded as follows: tree,s length: 237, consistency index (ci): 0.7721, and retention index (ri): 0.8666. in this tree, 15 populations with an exception of two outgroups were placed into two clades. genetic difference among the populations was estimated as 0-0.117 using the kimura-2 parameter model that explains a low level of genetic diversity and shows the investigated populations not being far apart from each other.

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تنوع ژنتیکی جمعیت‌های مختلف موریانه Microcerotermes diversus (Isoptera: Termitidae) با استفاده از ژن COII

موریانه Microcerotermes diversus (Isoptera: Termitidae) یک آفت مهم چوب در استان خوزستان بوده که خسارت‌های اقتصادی جدی را به لوازم سلولزی وارد می‌نماید. به منظور بررسی تنوع ژنتیکی این آفت، 13 جمعیت از نقاط مختلف استان خوزستان به علاوه 2 نمونه از جزیره خارک و بندرعباس جمع‌آوری گردید. بعد از استخراج ژنوم، یک قطعه با طول 486 جفت باز از ژن میتوکندریایی سیتوکروم اکسیداز دو (COII) تکثیر و سپس تعیین تو...

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